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Tumorigenic: Yes
Reverse Transcript: positive
Cytogenetic Analysis: modal number = 40; range = 32 to 80.
Mouse karyotype with a diploid stemline number of 40 chromosomes.
Age: 8 months
Gender: female
Comments: Tested and found negative for ectromelia virus （mousepox）.
The line was established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether. [22513]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 viable cells/ml.
Medium Renewal: Add fresh medium （20% to 30% by volume） every 2 to 4 days
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2040
References: 22513: . . J. Natl. Cancer Inst. 10: 179-192, 1949.
26055: . . J. Natl. Cancer Inst. 36: 405-421, 1966.
26056: . . Cancer Chemother. Rep. 51: 451-453, 1967.
32923: Young SW, et al. Gadolinium（III） texaphyrin: a tumor selective radiation sensitizer that is detectable by MRI. Proc. Natl. Acad. Sci. USA 93: 6610-6615, 1996. PubMed: 8692865
