MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2; MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2; MiaPaCa
貨號
XF0752
規格
1×10?cells/T25培養瓶
形態特征
?上皮細胞樣
生長特性
半貼壁生長
培養基
DMEM+10%FBS+2.5%HS 【MIA PaCa-2培養基有售，請聯系客服】
凍存條件
無血清細胞凍存液
培養條件
氣相：空氣，95%；CO2,5% 溫度：37℃
傳代方法
1:2傳代；
特征特性
DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%FBS
CRL-1420 MIA PaCa-2 人胰腺癌細胞 的介紹
ATCC® Number: CRL-1420™ Price: $279.00
Designations: MIA PaCa-2
Depositors: A Yunis
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, single cells and loosely attached clusters
Organism: Homo sapiens （human）
Morphology: attached epithelial with floating rounded cells
Source: Organ: pancreas
Disease: carcinoma
Cellular Products: human colony stimulating factor, subclass I （CSF-I）; plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory per
mits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1975
DNA Profile （STR）: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15
Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.
Isoenzymes: G6PD, B
Age: 65 years
Gender: male
Ethnicity: Caucasian
Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034]
The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034]
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207
References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590
22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line （MIA PaCa-2）. J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740
49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455
