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Chromosome Frequency Distribution 100 Cells: 2n = 60One large submetacentric marker chromosome. Most of the cells contained a chromosome with a secondary constriction and many of the cells contained dicentric chromosomes.
Comments:
The cells are positive for keratin by immunoperoxidase staining.
African green monkey kidney cell line suitable for transfection by SV40 vectors.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 3
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