人外周血淋巴細(xì)胞

CRL-8083 MC/CAR 人外周血淋巴細(xì)胞

ATCC® Number: CRL-8083™ Price: $417.00

Designations: MC/CAR

Depositors: Research Corporation

Isotype: IgG1 （cytoplasmic）; kappa light chain

Biosafety Leee Propagation

Growth Properties: suspension

Organism: Homo sapiens （human）

Morphology: lymphoblast

CRL-8083 MC/CAR 人外周血淋巴細(xì)胞vel: 2

Shipped: frozen

Medium & Serum: S

Source: Organ: peripheral blood

Disease: plasmacytoma; myeloma

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin

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Receptors: complement （C3b）

Tumorigenic: Yes

Antigen Expression: HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +

DNA Profile （STR）: Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,13

D16S539: 11,12

D5S818: 11,14

D7S820: 9,13

THO1: 6,9

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: normal male diploid

Isoenzymes: ADA, 1

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1

PGM3, 1

Age: 81 years

Gender: male

Ethnicity: Caucasian

Comments: Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]

The cells produce, but do not secrete, immunoglobulin.

No surface immunoglobulin.

8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.

Can be used as a human fusion line.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984

43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719

CRL-8083 MC/CAR