Biosafety Level: 2 [Cells containing SV40 viral DNA sequence ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: myofibroblast
CRL-2854 WPMY-1 人正常前列腺基質永生化細胞
Source: Organ: prostate
Tissue: stroma
Disease: normal
Cellular Products: fibronectin [89997]
smooth muscle alpha-actin
vimentin [89997]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1992
Receptors: androgen receptor, expressed （ [89997] upregulated upon exposure to androgen）
Tumorigenic: No
Antigen Expression: kallikrein 3, KLK3 （prostate specific antigen, PSA）; Homo sapiens [89997]
Cytogenetic Analysis: At passage 66, a majority of the cells were in the 58-68 range; X, -Y. [89997]
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 0
PGM1, 2
PGM3, 1
Age: 54 years
Gender: male
Ethnicity: Caucasian, White
Comments: The myofibroblast stromal cell line, WPMY-1, was derived from stromal cells from the same peripheral zone of the histologically normal adult prostate, as that used for RWPE-1 cells （ATCC CRL-11609）. Stromal cells were immortalized with SV40-large-T antigen gene, using a pRSTV plasmid construct. WPMY-1 stromal cells belong to a family of cell lines derived from the same prostate as the epithelial RWPE-1 cells and all of its epithelial derivatives. Because of their derivation from the same peripheral zone of the prostate, the WPMY-1 cell line is especially useful for studies on paracrine and stromal : epithelial interactions.
The depositor reports that the RWPE-1 cell line （ATCC CRL-11609）, derived from the same prostate, was screened for Hepatitis B and C, and human immunodeficiency viruses, and was found to be negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Growth Conditions: Subculture cells before or upon reaching confluence. Do not allow cells to become super-confluent.
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline （D-PBS）.
3.Add 2.0 to 3.0 ml of 0.025% Trypsin - 0.26 mM EDTA solution （1:1 dilution of 0.05% Trypsin - 0.53 mM EDTA in D-PBS） to the flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor （or 2% fetal bovine serum in D-PBS） and aspirate cells by gently pipetting.
5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.
6.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum between 4 X 10（3） to 6 X 10（3） viable cells/sq. cm is recommended.
7.Incubate cultures at 37C. We recommend that you maintain cultures at a cell concentraton between 6 X 10（3） and 5 X 10（4） cells/sq. cm.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 48 hours
Preservation: Freeze medium: Complete growth medium supplemented with an additional 15% fetal bovine serum and 10% （v/v） DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 38 hours
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2020
derived from same individual:ATCC CRL-11609
derived from same individual:ATCC CRL-11610
derived from same individual:ATCC CRL-2849
derived from same individual:ATCC CRL-2850
derived from same individual:ATCC CRL-2852
derived from same individual:ATCC CRL-2851
References: 46793: Bello D, et al. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18: 1215-1223, 1997. PubMed: 9214605
46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606
46799: Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420
46950: Okamoto M, et al. Interleukin-6 and epidermal growth factor promote anchorage-independent growth of immortalized human prostatic epithelial cells treated with N-methyl-N-nitrosourea. Prostate 35: 255-262, 1998. PubMed: 9609548
53180: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636
53181: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337
53182: Webber MM, et al. Immortalized and tumorigenic adult human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152
53183: Kremer R, et al. ras Activation of human prostate epithelial cells induces overexpression of parathyroid hormone-related peptide. Clin. Cancer Res. 3: 855-859, 1997. PubMed: 9815759
53192: Jacob K, et al. Osteonectin promotes prostate cancer cell migration and invasion: a possible mechanism for metastasis to bone. Cancer Res. 59: 4453-4457, 1999. PubMed: 10485497
53193: Achanzar WE, et al. Cadmium induces c-myc, p53, and c-jun expression in normal human prostate epithelial cells as a prelude to apoptosis. Toxicol. Appl. Pharmacol. 164: 291-300, 2000. PubMed: 10799339
53194: Achanzar WE, et al. Cadmium-induced malignant transformation of human prostate epithelial cells. Cancer Res. 61: 455-458, 2001. PubMed: 11212230
53196: Bello-DeOcampo D, et al. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46: 142-153, 2001. PubMed: 11170142
53197: Webber MM, et al. Human cell lines as an in vitro/in vivo model for prostate carcinogenesis and progression. Prostate 47: 1-13, 2001. PubMed: 11304724
89996: Quader ST, et al. Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model. Mutat. Res. 496: 153-161, 2001. PubMed: 11551491
89997: Webber MM, et al. A human prostatic stromal myofibroblast cell line WPMY-1: a model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20: 1185-1192, 1999. PubMed: 10383888
89998: Bello-DeOcampo D, et al. The role of alpha 6 beta 1 integrin and EGF in normal and malignant acinar morphogenesis of human prostatic epithelial cells. Mutat. Res. 480-481: 209-217, 2001. PubMed: 11506815
90372: Webber MM, et al. Modulation of the malignant phenotype of human prostate cancer cells by N-（4-hydroxyphenyl）retinamide （4-HPR）. Clin. Exp. Metastasis 17: 255-263, 1999. PubMed: 10432011
90375: Sharp RM, et al. N-（4-hydroxyphenyl）retinamide （4-HPR） decreases neoplastic properties of human prostate cells: an agent for prevention. Mutat. Res. 496: 163-170, 2001. PubMed: 11551492
90376: Carruba G, et al. Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells. Prostate 50: 73-82, 2002. PubMed: 11816015
