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Tumorigenic: Yes
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12,11.3
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Isoenzymes: AK-1, 1
ES-D, 1-2
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 67 years *
Gender: male
Comments: The HCT-8 line is identical to the HRT-18 cell line.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: RPMI 1640 medium with sodium pyruvate (1 mM); 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin, 0.02% EDTA in Hanks' BSS and incubate at 37C with the trypsin, EDTA for 10 to 15 minutes. Remove trypsin, EDTA, disperse cells in fresh medium, centrifuge, resuspend cells in medium and dispense into new flasks.
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
References: 1052: Tompkins WA, et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101-1110, 1974. PubMed: 4826581
26064: Nelson-Rees WA, et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975. PubMed: 1058173
32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293
