4T1 (ATCC® CRL-2539™) Organism Mus musculus, mouse Tissue mammary gland Product Format frozen4T1 小鼠乳腺癌細(xì)胞 Morphology epithelial Culture Properties adherent Biosafety Level 1 Disease This tumor is an animal stage IV human breast cancer. Strain BALB/cfC3H Applications The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer.
4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics. Storage Conditions liquid nitrogen vapor temperature Derivation 4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment. Tumorigenic Yes Effects 4T1 小鼠乳腺癌細(xì)胞 Yes, forms tumors and metastasizes in BALB/c mice Comments When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ. The primary tumor does not have to be removed to induce metastatic growth. Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.
Subculturing NOTE: the cells should not be allowed to become confluent, subculture at 80% of confluence. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature Culture Conditions Temperature: 37°C
